EVERYTHING ABOUT HYMENIDIN

The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products and solutions had been digested and cloned into a pET21a+ vector utilizing NdeI and XhoI restriction enzymes. A point mutation was launched by the quick modify web site-directed mut